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1. Specimens Due to the high stability of DNA, FISH can be performed on most specimens, ranging from blood and bone marrow smears, buccal smears, cytospins, and touch print preparations to archival pathology specimens and epithelial cells in bladder washings and urine. Logically, any nucleus can be evaluated with FISH methods as long as the DNA in the cell is not degraded (see Note 1). For hematological disorders, bone marrow (BM) and peripheral blood (PB) are usually the specimens submitted for FISH analysis.

Logically, any nucleus can be evaluated with FISH methods as long as the DNA in the cell is not degraded (see Note 1). For hematological disorders, bone marrow (BM) and peripheral blood (PB) are usually the specimens submitted for FISH analysis. Often these samples are first processed for chromosomal analysis and FISH is performed on the remaining fixed-cell pellet in cases of unsuccessful cytogenetics, to optimally interpret the observed abnormality or as a monitoring tool during treatment. BM is an ideal tissue for the observation of the in vivo chromosomal situation.

18 Sandberg and Chen References 1. Sandberg, A. A. (1990) The Chromosomes in Human Cancer and Leukemia, 2nd ed, Elsevier, New York. 2. LeBeau, M. M. (1991) Cytogenetic analysis of hematological malignant diseases, in The ACT Cytogenetics Laboratory Manual, 2nd ed. (Barch, M. ), Raven Press, New York, pp. 395–449. 3. , and Takino, T. (1986) Prefixation treatment with ethidium bromide for high resolution banding analysis of chromosomes from cultured human BM cells. Cancer Genet. Cytogenet. 22, 319–329.

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