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New PDF release: Advances in Lipid Methodology. Volume 3

By W. W. Christie

This can be the 3rd quantity of an occasional sequence of assessment volumes facing elements of lipid method. As with the 1st volumes, subject matters were chosen which have been constructing swiftly lately and feature a few significance to lipid research. The authors are all major overseas experts.

Topics coated contain: research of positional isomers of glycerolipids by means of non-enzymatic equipment, separation of phospholipid sessions by way of high-performance liquid chromatography, and nuclear magnetic resonance spectroscopy and lipid section behaviour and lipid diffusion, between others

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Extra resources for Advances in Lipid Methodology. Volume 3

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44. Kuksis,A. , Lipids, 1, 202-208 (1966). ADVANCES IN LIPID METHODOLOGY -THREE 35 45. Kuksis,A. , in Mass Spectrometry, pp. M. Lawson, Walter deGruyter, Berlin). 46. Kuksis,A. ,J. Chromatogr. (1995), in press. 47. , ltabshi,Y. , in Analysis of Fats, Oils and Lipoproteins, pp. G. Perkins, American Oil Chemists' Society, Champaign, IL). 48. , Yang,L-Y. , in Biological Mass Spectrometry, Present and Future, pp. 480-494 (1994) (edited by T. M. L. Gross and Y. Seyama, John Wiley, New York). 49. , in Advances in Lipid Methodology-One, pp.

This figure may be compared to earlier attempts to use 31 P NMR for phospholipid analysis. 1. 2. Effect ofphospholipid concentration on phosphorus chemical shifts The chemical shifts of some of the phospholipids vary to a small degree, depending on the concentration of total lipid in the reagent. These shift differences are particularly noticeable when analysis is attempted of extremely dilute ADVANCES IN LIPID METHODOLOGY -THREE 41 AAPC ""' EPLAS ""' JC DPG '\ LPE DHSM DPG AAPE PA LPS )'I LAAPC PG u GPLAS~ \ LPA- 1.

Further, signal intensity errors are consistent from sample to sample as long as the repetition rate and the solvent medium are not changed, permitting the use of calibration factors. With NMR, the advantage of using a high repetition rate in conjunction with calibration factors is that signalaveraging time can be reduced substantially without sacrificing analytical precision significantly. Factors that limit the accuracy of 31 P NMR measurements [1,2,5,75], 31 P NMR spectral profile measurements [35], and NMR measurements of extracted phos- 48 31 P NUCLEAR MAGNETIC RESONANCE PROFILING OF PHOSPHOLIPIDS pholipids [14,19,48,51,53,67] have been discussed.

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