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The slowing down of cell turnover (28 days for a young adult, 30 days on average for an older person) gives an impression of a dull complexion and thickened skin (horny layer), owing to the accumulation and overlapping of corneal cells, revealed by an aspect and feeling of cutaneous dryness, which is often the first complaint of an aging person. Impairment of the hydrolipoprotein layer and epidermal lipids contributes to the dehydration of the skin and feeling of discomfort. If this dryness is not corrected, wrinkles will more easily leave their imprint on the skin, with thinning of the ‘living’ epidermis (granular layer, stratum spinosum, and Malphighi layer) and irreversible modification of the dermoepidermal junction, which becomes flatter and loses support (impairment of collagen IV and VII and anchoring fibrils).

According to the method of evaluation used, the thickness and color of the replica may influence the quality of the measurement when an optical method is used. Mechanical microscopy technique This technique, based on the contact between a diamond tip and the surface, consists of scanning a line of microrelief, moving horizontally at a constant speed of around 1 mm/s. The diamond tip has a radius of curvature of 2 µm and exerts a pressure of about 10 mN on the surface. 1). To obtain one line, the detector moves along the x axis (perpendicular to the main axis of the cutaneous relief or wrinkle) in order to measure a certain length.

Qxd 11/11/2008 2:36 PM Page 33 Skin morphology and volume 33 With these new techniques, it is now possible to quantify objectively and accurately morphological changes of the skin surface during the aging process, after cosmetic injection, or after esthetic surgery. The precision of the analysis gives evidence for the influence of a cosmetic on the branches of the morphological tree by distinguishing the smoothing, restructuring, or thinning of lines and wrinkles. The current technological advances will help to disseminate the use of this technique in skin pathologies, probably usefully supplemented by video microscopy.

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