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Download PDF by Bernhard Sonnleitner: Bioanalysis and Biosensors for Bioprocess Monitoring

By Bernhard Sonnleitner

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So far direct electrochemical determination of cells does not seem to be very reliable [361]. Fuel cells [22] can measure the redox potential of a culture [149] but are comparably very slow and delayed. g. colony forming units (cfu), and to be rapid (a few minutes per analysis). This system was applied for on-line monitoring of an Escherichia coli culture. However, the dielectric properties of cells such as distinct deviations in the capacity values have been shown to depend on the growth stage of a culture [267].

However, the dielectric properties of cells such as distinct deviations in the capacity values have been shown to depend on the growth stage of a culture [267]. 3 Filtration Properties Lenz et al. [233] and Reuss et al. [344] have further developed an automatic filtration device [300, 435] that allows the estimation of the biomass concentration in a relatively large sample (approx. 100 ml) according to its filtration properties. Each sample is filtered through a fresh filter and the flux of the filtrate is monitored as well as the build-up of the filter cake.

G. [145, 317, 462, 463]. Often they concentrate especially on a certain group of biosensors but high redundancy is obvious. In the following sections, different sensor types, ordered by the transduction principle, are introduced. 32 B. Sonnleitner Fig. 22. Schematic design of one example of a biosensor, here an optical biosensor or “opt(r)ode”. The general working principle is that a biological catalyst/recognition element is tightly coupled with a so-called transducer. In this special case, the transducer is an optical system, namely optical fibers connected to a light source and a detector (indicated with L and D).

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