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David W. Burden, Donald B. Whitney (auth.)'s BiotechnologyProteins to PCR : A Course in Strategies and PDF

By David W. Burden, Donald B. Whitney (auth.)

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Extra info for BiotechnologyProteins to PCR : A Course in Strategies and Lab Techniques

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Although each species of protein possesses the same amino acid sequence, proteins are considered heterogeneous polymers because the amino acids which comprise the sequence of the protein are not identical. 1). 2 (A) Peptide bond formation. (B) Three dimensional view of dipeptide showing planar peptide bond. All biomolecules are essential in their own right, but proteins take on special importance due to the versatility of their biological functions. Proteins regulate metabolic activity, catalyze the biochemical reactions necessary for proper cell function, and maintain the structural integrity of the cell and organism.

YNB galactose medium is prepared accordingly. Streak Plating of Escherichia coli and Saccharomyces carlsbergensis Streaking microorganisms is routine in the biotechnology laboratory. Streak plating employs aseptic technique. Do not touch or needlessly expose any sterile item to a nonsterile item, especially AIR. 5% yeast extract, and 2% agar in water. Sterilize by autoclaving and aseptically pour into disposable plastic petri dishes. Five plates can easily be poured from 100 ml of liquified LB agar.

If the needle is still hot from the flame it can first be cooled by touching it to a sterile area of the agar. 4. Remove the foil from the YPD flask, remove the plug (grasp it in the center so as not to contaminate the lip of the flask), and insert the tip of the loop (with the cells) into the broth. Do not touch the sides of the flask with the loop as you cannot guarantee it is sterile. Remove the loop and plug the flask. During the inoculation, the plug should Chapter 1 • Introduction to the Biotechnology Laboratory 29 never be placed on the bench.

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