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Chemical Derivatization in Liquid Chromatography - download pdf or read online

By J.F. Lawrence and R.W. Frei (Eds.)

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4 Chromatography apparatus The most common method used for TLC is the ascending technique in closed chambers containing the mobile phase. The solvent is permitted to flow up the plate (by capillary action) until the desired separation is achieved. Chromatography chambers are available in many sizes and shapes. 2). These tanks can also accommodate plates of dimensions 10 X 20 cm and 5 X 20 cm, although smaller tanks are available. Special racks may be obtained which can accommodate 12 or more upright TLC plates in the chromatography chamber for simultaneous analysis.

Because one ionizing particle entering the detector can ultimately produce a large number of ions in the gas (as much as a lo7-lo9 fold increase), external amplification is not required. Proportional counters are similar in principle to the Geiger-Muller counter, but differ in that the voltage potential across the electrodes is much less. This reduction in voltage decreases the large amplification effect of the GeigerMuiler counter to the extent that the current produced is proportional to the number of ions created by the original ionizing particle.

However, the technology of commercial TLC layers and reflectance instruments has improved to such an extent that quantitative results can be readily obtained with reasonable precision. 11 shows a scan of a chromatogram of some nucleotides separated on purified cellulose [45]. A comparison of reflectance and transmission in absorption measurements is of interest Hypmnthinc x - @T""ine - 2. Dimension Fig. 1 1. Thin-layer chromatogram and corresponding peaks for some nucleotides separated on purified cellulose.

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